[ccp4bb] Apperance of ice ring in cryo after addition of protein crystal ( otherwise absent in only cryo)

- Protein crystallography

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- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-09-01

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From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-09-01

Subject: Apperance of ice ring in cryo after addition of protein crystal ( otherwise absent in only cryo)
From: arpita goswami bt {- dot -} arpita {- at -} GMAIL {- dot -} COM
Date: 2009-09-01

Hi all,

A non CCP4 question, the cryo diffraction without protein crystal had
absolutely no ice ring. But when I transferred protein crystal from the
sitting drop to the cryo, there was ice ring in diffraction pattern.

My doubt is whether water shelled around the crystal led to this ice ring,
and how to remove it? Actually siiting drop was set in microbatch plate
layered with around 2.5 ml paraffin oil. Was this oil anyway responsible for
this water shell to be kapt on the crystal?

Crystallization condition: 20% Jeffamine M-600, 0.1M HEPES (pH 7.5). Cryo
condition: 20% Jeffamine M-600, 0.1M HEPES (pH 7.5), 15% glycerol.

Regards,

Arpita.
---
Arpita Goswami
Junior Research Fellow
Structural Biology Laboratory
Centre for DNA Fingerprinting and Diagnostics (CDFD)
Hyderabad

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: cracking crystal
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-09-01

Next message:
Subject: Re: space groups not supported by Refmac5
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-09-01