Re: [ccp4bb] cracking crystal

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anisotropic diffraction in refinement
From: Lari Lehtiö lari {- dot -} lehtio {- at -} ABO {- dot -} FI
Date: 2009-09-01

Next message:
Subject: Re: cracking crystal
From: Eric Larson larsonet {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-09-01

Subject: Re: cracking crystal
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-09-01

You can always give this one a try:

http://www.xtals.org/crystal_cryo.pdf

Artem

"Nothing is built on stone; all is built on sand, but we must build as if
the sand were stone"

Jorge Luis Borges

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Fengxia Liu
Sent: Tuesday, September 01, 2009 5:25 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cracking crystal

Dear All,

Could you please help me solve this problem?

I have a sensitive crystal, the mother liquor is 10% PEG4k+ 100 mM
Tris-buffer pH 8.5, crystal is big and good but very sensitive, when i put
it in cryoprotectants, cracking happened. First i used artificial mother
liquor + 25% v/v glycerol, slowly decrease to + 12.5% v/v glycerol, all
crystals cracked after immersed, finally i tried 50% mineral oil + 50%
paratone, it still cracked (4- 5 cracks, but not broken) . even this cracked
crystal can give me 3.2 angstrom diffraction, so no cracked crystal might
give me 2.0+ angstrom diffraction. Now i don't have many crystals to try so
many cryoprotectants, so anybody has experience on this? any suggestion
would be greatly appreciated.

Thank you.

Fengxiale

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anisotropic diffraction in refinement
From: Lari Lehtiö lari {- dot -} lehtio {- at -} ABO {- dot -} FI
Date: 2009-09-01

Next message:
Subject: Re: cracking crystal
From: Eric Larson larsonet {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-09-01