Re: [ccp4bb] Cold Cabinet Vs Fridge, FPLC

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Cold Cabinet Vs Fridge, FPLC
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-09-01

Some proteins can be purified at room temperature. Others would be degraded
or may lose 'competency' (however you wish to define the latter). As a rule
of thumb, *most* proteins will not be hurt by exposure to +4C, but e.g.
detrimental protease activity should follow the normal kinetic rules and
slow down considerably at +4C as opposed to +21C. There are many options out
there and it's hard to anticipate all eventualities - a cold-capable
purification system is not a luxury* but an essential tool in any protein
lab.

Artem

* this is a very clumsy reference to the immortal words of Ostap Bender from
the book 'The Little Golden Calf'. People familiar with early Soviet
literature will get the reference.

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ho
Leung Ng
Sent: Monday, August 31, 2009 11:31 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Cold Cabinet Vs Fridge, FPLC

I know several labs that keep their FPLCs at room temperature. Maybe
the cold cabinet isn't necessary?

ho

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: MAMA-mask
From: Xiaowei Pan panxiaow {- at -} GMAIL {- dot -} COM
Date: 2009-09-01

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Subject: Re: Off Topic: Stereo Microscope Recommendations
From: kaiser {- at -} CALTECH {- dot -} EDU kaiser {- at -} CALTECH {- dot -} EDU
Date: 2009-09-02