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Re: [ccp4bb] MAMA-mask |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: MAMA-mask From: Kevin Cowtan cowtan {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK Date: 2009-09-02 You need to run mapmask to extend the mask to cover an assymetric unit using the overlap option. CCP4i instruction here: http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=Electron_density_manipulation#Converting_a_non-crystallographic_mask_to_a_crystallographic_mask This is becoming a very FAQ. I guess I need to do some magic in Coot to at least warn people when their map is going to give problems. Kevin Xiaowei Pan wrote: > > > Hello all > > I've made a mask by MAMA from a PDB file. I also used command "Atom_fit" > to check that all of the atoms were covered by the mask. > But after converting the mask to ccp4 format and opening in COOT, the > mask was too small, only covered half of the model. The mask was cut > along two axis. > I cannot find out the reason. > > Thanks for your help! > > Xiaowei > > > > > 2009-09-02 > Xiaowei Pan CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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