Re: [ccp4bb] Weird expression behavior

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Dianfan Li dianfan {- dot -} li {- at -} UL {- dot -} IE
Date: 2009-09-02

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Subject: Re: Weird expression behavior
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2009-09-02

Subject: Re: Weird expression behavior
From: Ezra Peisach epeisach {- at -} GMAIL {- dot -} COM
Date: 2009-09-02

A little tangental.... You mentioned lowering the temperature - how
low... Stratagene markets a call line - Arctic Express - that adds
chaperones that are more active at lower temps. I know someone who
overcame inclusion body problems by expression at 16 using these cells.
(I know someone else who regularly induces o/n at 16C w/ regular
cells). The only draw back is that these cells produce a protein that
binds to NiNTA resin...

Good luck...

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Thanks Re: Cold Cabinet Vs Fridge, FPLC
From: Dianfan Li dianfan {- dot -} li {- at -} UL {- dot -} IE
Date: 2009-09-02

Next message:
Subject: Re: Weird expression behavior
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2009-09-02