Re: [ccp4bb] Weird expression behavior

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Weird expression behavior
From: Ezra Peisach epeisach {- at -} GMAIL {- dot -} COM
Date: 2009-09-02

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Subject: Re: Rfactor got stuck with a data having alternate strong and weak reflections.
From: Yuan Cheng ycheng {- at -} EMAIL {- dot -} UNC {- dot -} EDU
Date: 2009-09-02

Subject: Re: Weird expression behavior
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2009-09-02

Hi Ezra and others,

Just thought I'd let you know that I have noticed that one or two of
those E. coli proteins that love to bind Ni-affinity resin do not
bind to Cobalt resin. Of course, there is always a price to pay (for
the Co resin, in this case) :)!

Cheers,
Raji

-----------
Raji Edayathumangalam
Joint Research Fellow
Brigham and Women's Hospital/
Harvard Medical School
Brandeis University

On Sep 2, 2009, at 7:55 AM, Ezra Peisach wrote:

> A little tangental.... You mentioned lowering the temperature -
> how low... Stratagene markets a call line - Arctic Express - that
> adds chaperones that are more active at lower temps. I know
> someone who overcame inclusion body problems by expression at 16
> using these cells. (I know someone else who regularly induces o/n
> at 16C w/ regular cells). The only draw back is that these cells
> produce a protein that binds to NiNTA resin...
>
> Good luck...

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Weird expression behavior
From: Ezra Peisach epeisach {- at -} GMAIL {- dot -} COM
Date: 2009-09-02

Next message:
Subject: Re: Rfactor got stuck with a data having alternate strong and weak reflections.
From: Yuan Cheng ycheng {- at -} EMAIL {- dot -} UNC {- dot -} EDU
Date: 2009-09-02