[ccp4bb] anaerobic crystallization

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Staff Scientist and 2 Research Technician positions in Vienna
From: Kristina Djinovic Carugo kristina {- dot -} djinovic {- at -} UNIVIE {- dot -} AC {- dot -} AT
Date: 2009-09-02

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Subject: Re: Refmac Problem - Permission Denied
From: David Jones David {- dot -} Jones {- at -} UCDENVER {- dot -} EDU
Date: 2009-09-02

Subject: anaerobic crystallization
From: Christopher Rife christopher {- dot -} rife {- at -} DANISCO {- dot -} COM
Date: 2009-09-02

Hi,

Does anyone have tips or suggestions for getting started with anaerobic
crystallization? Searching through the archives, I was able to find some
discussion about various glove box options
(http://tinyurl.com/ccp4-glovebox), but not about the process itself (we
already have a box).

To simplify things, would it be worthwhile to perform the initial screens
in something like the pre-filled Qiagen screens
(http://tinyurl.com/qiagen-xseal)? Do I need to worry about evaporation
while solutions are being degassed (esp volatiles)? Better/easier to try
microbatch?

Thanks for any input.

Chris
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Refmac Problem - Permission Denied
From: David Jones David {- dot -} Jones {- at -} UCDENVER {- dot -} EDU
Date: 2009-09-02