[ccp4bb] problem in expression of glycoproteins in baculovirus expression system

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Rfactor got stuck with a data having alternate strong and weak reflections.
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-09-03

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Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Shao-Yang Ku s {- dot -} ku {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-09-03

Subject: problem in expression of glycoproteins in baculovirus expression system
From: ruheng rh_ibp2006 {- at -} HOTMAIL {- dot -} COM
Date: 2009-09-03

Dear all,

Recently I am secretely expressing an ectodomain of a human membrane protein in insect cells. The expression level is moderately high but the target band in the SDS-PAGE gel seems smearing and this is cosistent with the western blot results. Does it result from the heterogeneous glycosylation of the protein? My protein contains quite a lot of N and O linked polysaccharide chains and they are important for the function of the protein. Does the heterogeneously glycosylated protein affect the crystallization? Does anyone have the experience of crystallizing such kind of glycoprotein?

Thanks in advance!

Ru Heng

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Shao-Yang Ku s {- dot -} ku {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-09-03