Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problem in expression of glycoproteins in baculovirus expression system
From: ruheng rh_ibp2006 {- at -} HOTMAIL {- dot -} COM
Date: 2009-09-03

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Subject: Re: Problem in MAKE U POSITIVE
From: Martyn Winn martyn {- dot -} winn {- at -} STFC {- dot -} AC {- dot -} UK
Date: 2009-09-03

Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Shao-Yang Ku s {- dot -} ku {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-09-03

Can you put a 6His-tag on your protein and add some Ni-NTA beads to
your solution to capture (and concentrate) the properly folded
molecules?

Quoting "Xuan Yang" :

> Dear All,
>
> I am working on protein refolding via dialysis in large volumn
> (typically 2~4 litters). It was problematic when I wanted to concentrate the
> solution to at least less than 500ml. If you know any device appropriate for
> such task, please help me out:)
>
> Thanks in advance!
>
> Sincerely,
>
> Xuan Yang
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problem in expression of glycoproteins in baculovirus expression system
From: ruheng rh_ibp2006 {- at -} HOTMAIL {- dot -} COM
Date: 2009-09-03

Next message:
Subject: Re: Problem in MAKE U POSITIVE
From: Martyn Winn martyn {- dot -} winn {- at -} STFC {- dot -} AC {- dot -} UK
Date: 2009-09-03