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Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Problem in MAKE U POSITIVE
From: Martyn Winn martyn {- dot -} winn {- at -} STFC {- dot -} AC {- dot -} UK
Date: 2009-09-03		Next message:
Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: "Demetres D {- dot -} Leonidas" ddl {- at -} EIE {- dot -} GR
Date: 2009-09-03


Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Xuan Yang pattisyang {- at -} GMAIL {- dot -} COM
Date: 2009-09-03

Dear Dr Ku,

It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg
and 1mM EDTA.

And I want to try other affinity columns, just don't know what resin would
be appropriate.

Sincerely,

Xuan Yang


2009/9/3 Shao-Yang Ku

> Can you put a 6His-tag on your protein and add some Ni-NTA beads to your
> solution to capture (and concentrate) the properly folded molecules?
>
>
> Quoting "Xuan Yang" :
>
> Dear All,
>>
>> I am working on protein refolding via dialysis in large volumn
>> (typically 2~4 litters). It was problematic when I wanted to concentrate
>> the
>> solution to at least less than 500ml. If you know any device appropriate
>> for
>> such task, please help me out:)
>>
>> Thanks in advance!
>>
>> Sincerely,
>>
>> Xuan Yang
>>
>>
>
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