Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Xuan Yang pattisyang {- at -} GMAIL {- dot -} COM
Date: 2009-09-03

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From: Tobias Krojer Tobias {- dot -} Krojer {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-09-03

Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: "Demetres D {- dot -} Leonidas" ddl {- at -} EIE {- dot -} GR
Date: 2009-09-03

Dear Xuan Yang,

for such large volumes we use a Pellicon type system. The one we have in
the lab is a polyethersulfone PTJK prep/Scale TFF 2.5 FT2 High flux 10 k
from Millipore.

good luck

Demetres

Xuan Yang wrote:
> Dear All,
>
> I am working on protein refolding via dialysis in large volumn
> (typically 2~4 litters). It was problematic when I wanted to
> concentrate the solution to at least less than 500ml. If you know any
> device appropriate for such task, please help me out:)
>
> Thanks in advance!
>
> Sincerely,
>
> Xuan Yang

--
Demetres D. Leonidas, Ph.D.
Senior Researcher
Structural Biology & Chemistry Group
Institute of Organic and Pharmaceutical Chemistry
The National Hellenic Research Foundation
48, Vassileos Constantinou Avenue
Athens 116 35, Greece
==================================================
Tel. +30 210 7273841 (office)
+30 210 7273895 (lab)
Fax. +30 210 7273831
E-mail: ddl@eie.gr
URL: http://athena.eie.gr
==================================================

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale
From: Xuan Yang pattisyang {- at -} GMAIL {- dot -} COM
Date: 2009-09-03

Next message:
Subject: saint to scala
From: Tobias Krojer Tobias {- dot -} Krojer {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-09-03