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Re: [ccp4bb] How to improve the Se-Met crystal diffracted to 5.6A |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: How to improve the Se-Met crystal diffracted to 5.6A From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG Date: 2009-09-03 My suggestion would be to attempt finding Se atoms in the MAD dataset and then use these coordinates to do sulphur phasing - assuming of course that you can get the native crystals to diffract at an appropriately low-energy X-ray source. Artem > > Dear all, > > > > I have a Se-Met crystal diffracted to 5.6A and a full data set of the same > native crystal diffracted to 2.8A. Since I can hardly improve the quality > of the Se-Met crystal, may I ask some suggestion from the community about > am I able to solve the structure based on the above resolution, and how? > > > > Zhen > > _________________________________________________________________ > Show them the way! Add maps and directions to your party invites. > http://www.microsoft.com/windows/windowslive/products/events.aspx CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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