Re: [ccp4bb] How to get rid of the skin in the screening drop

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: stabilizing solution
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Date: 2009-09-06

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Subject: Re: How to get rid of the skin in the screening drop
From: Shu XU xushuhere {- at -} GMAIL {- dot -} COM
Date: 2009-09-06

Subject: Re: How to get rid of the skin in the screening drop
From: Jeremiah Farelli jfarelli {- at -} BU {- dot -} EDU
Date: 2009-09-06

This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration to
7.5 mg/mL... the crystals still grew, and the skin didn't.

This seems to happen a lot when you set up crystals with PEGs, so you might
be able to lower the concentration of PEG as well.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: stabilizing solution
From: james09 pruza james09xtal {- at -} GMAIL {- dot -} COM
Date: 2009-09-06

Next message:
Subject: Re: How to get rid of the skin in the screening drop
From: Shu XU xushuhere {- at -} GMAIL {- dot -} COM
Date: 2009-09-06