[ccp4bb] Help with improving these crystals

- Protein crystallography

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- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Team Leader Position at EMBL, Hamburg Unit
From: Margret Fischer margret {- dot -} fischer {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-09-09

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Subject: Re: Help with improving these crystals
From: Jim Fairman fairman {- dot -} jim {- at -} GMAIL {- dot -} COM
Date: 2009-09-09

Subject: Help with improving these crystals
From: Narayanan Ramasubbu ramasun1 {- at -} UMDNJ {- dot -} EDU
Date: 2009-09-09

Dear All:
We have been struggling to improve the crystals shown in the attachment.
These crystals form from a protein solution 8 mg/mL (before drop), drop
sizes are 2 microL of protein + 2 microL of well solution (0.1 MES
buffer, pH 6.5, 1.8 M Ammonium sulfate with 12 mM CoCl2).

The protein was dissolved initially in 20 mM Tris.HCl, pH 8.0 containing
1 mM EDTA.

Low cobalt also gives smaller crystals. These diffract up to 8 A.

Any suggestions to improve these crystals would be most welcome.

Subbu

PS: We have used many additives (Hampton Research - including detergent
additives) to alter the morphology but with little success.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Team Leader Position at EMBL, Hamburg Unit
From: Margret Fischer margret {- dot -} fischer {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-09-09

Next message:
Subject: Re: Help with improving these crystals
From: Jim Fairman fairman {- dot -} jim {- at -} GMAIL {- dot -} COM
Date: 2009-09-09