Re: [ccp4bb] Preparation of seed-stocks without seed-beads

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Preparation of seed-stocks without seed-beads
From: Ramanathan Natesh r {- dot -} natesh {- at -} MAIL {- dot -} CRYST {- dot -} BBK {- dot -} AC {- dot -} UK
Date: 2009-09-12

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From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2009-09-12

Subject: Re: Preparation of seed-stocks without seed-beads
From: "Snyder, Greg (NIH/NIAID) [F]" GSNYDER {- at -} NIAID {- dot -} NIH {- dot -} GOV
Date: 2009-09-12

We used to use another glass cover slip and make a sandwich with the drop and rub together. Recovery is a bit messy though.

Greg Snyder, Ph.D.
Postdoctoral Fellow
Laboratory of Immunology
Structural Immunobiology Section
National Institute of Allergy and Infectious Diseases
National Institutes of Health
Building 4, Room 138
4 Center Drive
Bethesda, MD 20892-0430
U.S.A.

Phone: 301-402-7523
FAX: 301-480-1291
E-mail: gsnyder@niaid.nih.gov
http://www3.niaid.nih.gov/labs/aboutlabs/li/StructuralImmunobiology/
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-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ramanathan Natesh
Sent: Saturday, September 12, 2009 10:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Preparation of seed-stocks without seed-beads

Hi
Most probably you might have micro seeds stock in your present well.
A streak with cat whisker/something like that in your old well and immedietly a streak in your new well might work miracles sometimes.
HTH
all the best
Natesh.

>
> >> Dear CCP4bbers,
> >>
> >> Can anyone suggests how to make seed-stocks if one is not having
> >> seed-beads... Is there any other methods to crush the crystals for
> >> the same purpose. What if it is simple vortexed. Off-course there
> >> wold be all sorts of sizes, the intact crystals as well.
> >> Please suggest.
> >>
> >> Thanks a lot more for previous help.
> >> James.
>
>

---------------------------------------
Ramanathan Natesh
Researcher ID: C-4488-2008 (http://www.researcherid.com/rid/C-4488-2008)
Wellcome Trust Advanced Training Fellow
School of Crystallography
Birkbeck College
University of London
Malet Street
London WC1E 7HX
Tel: +44 (0)20-7631-6850/6824
Fax: +44 (0)20-7631-6803
---------------------------------------
"Live Simply and do Serious Things .. "
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"In Science truth always wins"
- Max Ferdinand Perutz OM FRS
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Off topic: Question about CD spectrum
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2009-09-12