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Re: [ccp4bb] Using SAXS data for phasing at mediocre resolution.
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Preparation of seed-stocks without seed-beads
From: Ed Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2009-09-14		Next message:
Subject: Re: Using SAXS data for phasing at mediocre resolution.
From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2009-09-14


Subject: Re: Using SAXS data for phasing at mediocre resolution.
From: mjvdwoerd {- at -} NETSCAPE {- dot -} NET mjvdwoerd {- at -} NETSCAPE {- dot -} NET
Date: 2009-09-14


Hi Francis,

There is an older paper that mentions this idea: Tsao et al, Acta Cryst B48 (1992), 75-88. However, when you look at the paper, small-angle scattering data is not the only thing that was used. In particular, if my memory serves me right, the 60-fold averaging applied to the problem really made all the difference in getting the phases right.
The big difference that works to your advantage is that small-angle scattering was not nearly as well developed at the time as it is now. For example, I don't think they could get molecular envelopes in 1992. There is sufficient information available these days that the test could be done (for appropriate systems).

Mark







-----Original Message-----
From: Francis E Reyes
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, Sep 14, 2009 9:47 am
Subject: [ccp4bb] Using SAXS data for phasing at mediocre resolution.









Hi all?
?

I'm looking for anyone who has had (practical) experience using SAXS data to phase 4.2 A crystals. Please email me.?
?

FR?
?

---------------------------------------------?

Francis Reyes M.Sc.?

215 UCB?

University of Colorado at Boulder?
?

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