Re: [ccp4bb] cryosolvent

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: cryosolvent
From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2009-09-19

Next message:
Subject: Regarding CCP4 on mac problem solved
From: Mohinder Pal mp11 {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2009-09-20

Subject: Re: cryosolvent
From: Elspeth Garman elspeth {- dot -} garman {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-09-19

That is a heavy PEG, and they arent such good cryo-agents. If you get
ice-rings when you test your buffer aone, try adding 15% of a much
lighter PEG (400 or 600).
Happy cryo-cooling
Elspeth

Roger Rowlett wrote:
> With that much PEG in your mother liquor, I don't think you will need
> any cryosolvent. It will likely vitrify adequately in LN2 directly
> from this solution. In solutions that do not contain high
> concentrations of PEG or polyols, 25-30% glucose is nearly always
> sufficient, and is compatible with high salt concentrations.
>
> Cheers.
>
>
> rui wrote:
>> Hi, All,
>> What's the general rules for selecting cryosolvent?I got crystals in 30%
>> PEG4000/PEG3350, 0.2M AS and 0.1M NaAC,how should I choose cryosolvent?
>> Thanks.
>>
>> Rui
>>
>>
> --
> ------------------------------------------------------------------------
> Roger S. Rowlett
> Professor
> Department of Chemistry
> Colgate University
> 13 Oak Drive
> Hamilton, NY 13346
>
> tel: (315)-228-7245
> ofc: (315)-228-7395
> fax: (315)-228-7935
> email: rrowlett@colgate.edu

--
******NOTE NEW E-MAIL ADDRESS AND PHONE NUMBER************
----------------------------------------------------
Elspeth F. Garman,
President, British Crystallographic Association
Professor of Molecular Biophysics, University of Oxford
Visiting Professor in Chemistry, University of Durham
Postal address:
Laboratory of Molecular Biophysics,
Department of Biochemistry,
University of Oxford, Tel: (44)-1865-613297
South Parks Road, FAX: (44)-1865-613201
OXFORD, OX1 3QU, U.K. E-mail: elspeth.garman@bioch.ox.ac.uk
http://www.biop.ox.ac.uk/www/garman/gindex.html
-----------------------------------------------------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: cryosolvent
From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2009-09-19

Next message:
Subject: Regarding CCP4 on mac problem solved
From: Mohinder Pal mp11 {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2009-09-20