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Re: [ccp4bb] taming structural biology |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: taming structural biology From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK Date: 2009-09-28 I like the David Blow book very much Outline of Crystallography for Biologists /by David Blow And there is another good one by Jan Drenth.. Eleano Katja Schleider wrote: > Hello everyone, > > I am no structural biologist but a microbiologist. I have a cooperation with a crystallographic group to solve the structure of a bacterial enzyme. Now we have recorded a dataset and are solving the structure. My problem is that the whole Structural Biologist thing is so complex (Biology, chemistry physics...). Nevertheless its very fascinating. But what I want to know is, if there is a good textbook for it. I got "crytallography made crystal clear" but this book isn't enough at all. Especially for data processing. I want to know things like which values are good for Rfactor observerd/expected or for R-meas Rmrgd-F after the correctstep with xds. Is 80% completness still ok? And after scaling we had a Rmerge of 0.156 (overall; at 2.2 A). > My pHD-cooperation partner isn't that experienced as well, so its difficult to understand everything and do everything correct. > I hope these questions are ok and somebody can help with a textbook or publication. > > Thanks in advance, > > Katja > > > > CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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