Re: [ccp4bb] coiled coil question

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: gpp4 version 1.2 released
From: "William G {- dot -} Scott" wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2009-10-05

Next message:
Subject: Post-doctoral position in Biochemistry and Structural Biology with emphasis on Antioxidant Proteins
From: Karen Fulan Discola kdiscola {- at -} GMAIL {- dot -} COM
Date: 2009-10-05

Subject: Re: coiled coil question
From: Xie Jiabao xie {- dot -} jiabao {- at -} YAHOO {- dot -} COM
Date: 2009-10-05

Dear all,

I thank everyone who directed me to coiled coil structures where the alpha helical chains are related by a crystallographic axis. My question was prompted by an examination of structures (mainly parallel homodimers) where this is not the case. In most structures, including 2zta (the classic GCN4 leucine zipper dimer), a strict 2 fold does not exist. In an instance such as 2zta, is the 'small' deviation from a 2-fold due to the absence of crystallographic symmetry restraints? Also, in a structure such as tropomyosin, not only is there a deviation from the 2-fold but the two chains themselves are not structurally equivalent.

Best regards,
Xie

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: gpp4 version 1.2 released
From: "William G {- dot -} Scott" wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2009-10-05