[ccp4bb] Reducing Agent Tips?

- Protein crystallography

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- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: anisotropic data
From: Katja Schleider katjaschleider {- at -} YAHOO {- dot -} DE
Date: 2009-10-05

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Subject: Re: Reducing Agent Tips?
From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2009-10-05

Subject: Reducing Agent Tips?
From: Jeremiah Farelli jfarelli {- at -} BU {- dot -} EDU
Date: 2009-10-05

Hello all,

Anyone have any tips for reducing agents for use with the following
crystallization conditions:

100 mM hepes, pH 7.5, 24% PEG 1500

or

100 mM Tris, pH 8.0, 24% PEG 1500

I've tried BME and DTT (1 mM). Currently trying out TCEP. The protein
seems to be sensitive to oxidation, with a selenomet-derivative even more so.

Thanks!

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: anisotropic data
From: Katja Schleider katjaschleider {- at -} YAHOO {- dot -} DE
Date: 2009-10-05

Next message:
Subject: Re: Reducing Agent Tips?
From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2009-10-05