[ccp4bb] Mounting needle-shaped crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anisotropic data
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-10-05

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From: Ho Leung Ng ho {- at -} CONFOMETRX {- dot -} COM
Date: 2009-10-05

Subject: Mounting needle-shaped crystals
From: "Tanner, John J {- dot -} " TannerJJ {- at -} MISSOURI {- dot -} EDU
Date: 2009-10-05

Dear CCP4,

I'm looking for advice on mounting thin needles for low temperature data
collection. Our needles are fairly long (100-200 microns) but only 20
microns or less thick. When I pick them up with Hampton loops (0.05-0.1 mm
size), the crystals tend to break as they are moved out of the drop and
through the liquid-air interface.

I see that Mitegen sells MicroLoops E, which are advertised as working well
for mounting needles. Can anyone recommend them? Can anyone recommend
Mitegen MicroMeshes or another tool for mounting needles?

Thanks,

Jack Tanner

--
John J. Tanner
Professor of Chemistry and Biochemistry
University of Missouri-Columbia
125 Chemistry Building
Columbia, MO 65211
Phone: 573-884-1280
Fax: 573-882-2754
Email: tannerjj@missouri.edu
http://www.chem.missouri.edu/TannerGroup/tanner.html

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anisotropic data
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-10-05

Next message:
Subject: Re: Reducing Agent Tips?
From: Ho Leung Ng ho {- at -} CONFOMETRX {- dot -} COM
Date: 2009-10-05