Re: [ccp4bb] Mounting needle-shaped crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Mounting needle-shaped crystals
From: Charlie Bond Charles {- dot -} Bond {- at -} UWA {- dot -} EDU {- dot -} AU
Date: 2009-10-05

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Subject: Re: anisotropic data
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-10-06

Subject: Re: Mounting needle-shaped crystals
From: jpm premo {- at -} BIOXRAY {- dot -} AU {- dot -} DK
Date: 2009-10-06

Dear Jack
I can certainly recommend using the litholoops from molecular dimensions
to mount the crystal you descibe. Most of the membrane crystal we start
out with come as needles, the advantages is as mention before that the
slight twist in the nylon loops isn't present. We do not have much
success with the litholoops looking like little rackets.
best Preben

Tanner, John J. wrote:
> Dear CCP4,
>
> I'm looking for advice on mounting thin needles for low temperature data
> collection. Our needles are fairly long (100-200 microns) but only 20
> microns or less thick. When I pick them up with Hampton loops (0.05-0.1 mm
> size), the crystals tend to break as they are moved out of the drop and
> through the liquid-air interface.
>
> I see that Mitegen sells MicroLoops E, which are advertised as working well
> for mounting needles. Can anyone recommend them? Can anyone recommend
> Mitegen MicroMeshes or another tool for mounting needles?
>
> Thanks,
>
> Jack Tanner
>
>
>

--
Jens Preben Morth, Ph.D
Aarhus University
Department of Molecular Biology
Gustav Wieds Vej 10 C
DK - 8000 Aarhus C
Tel. +45 8942 5025, Fax. +45 8612 3178
jpm@mb.au.dk
website: http://person.au.dk/da/jpm@mb

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Mounting needle-shaped crystals
From: Charlie Bond Charles {- dot -} Bond {- at -} UWA {- dot -} EDU {- dot -} AU
Date: 2009-10-05

Next message:
Subject: Re: anisotropic data
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-10-06