[ccp4bb] Phase separation to crystals

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: monomer library for stereoisomers ?
From: "Mueller, Juergen-Joachim" jjm {- at -} MDC-BERLIN {- dot -} DE
Date: 2009-10-17

Next message:
Subject: Re: Phase separation to crystals
From: Frederic VELLIEUX frederic {- dot -} vellieux {- at -} ORANGE {- dot -} FR
Date: 2009-10-17

Subject: Phase separation to crystals
From: james09 pruza james09xtal {- at -} GMAIL {- dot -} COM
Date: 2009-10-17

Dear CCP4bbers,

Surprisingly, only At pH 3.6 the needles are appearing after 3 days but
they are very thin and long rods. I have checked it running SDS-PAGE as well
as using IZIT dye . Since the proteins has already 50mM of NaCl so I added
100mM of NaCl in the crystallization mix. The crystal appeared at 70% of
MPD. Bunch of florets appears without NaCl at the same condition. Will the
Additives like Ethanol or 2-propanol help in this condition? Should I not
use NaCl in the mix? Changing temperature from 4degree to 25 degree will
help?
Please advice.

Thanks a lot.
James

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: monomer library for stereoisomers ?
From: "Mueller, Juergen-Joachim" jjm {- at -} MDC-BERLIN {- dot -} DE
Date: 2009-10-17

Next message:
Subject: Re: Phase separation to crystals
From: Frederic VELLIEUX frederic {- dot -} vellieux {- at -} ORANGE {- dot -} FR
Date: 2009-10-17