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[ccp4bb] Difficult molecular replacement in R32 with pseudo translation
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: how to improve Rfree?
From: Bart Hazes Bart {- dot -} Hazes {- at -} UALBERTA {- dot -} CA
Date: 2009-10-19		Next message:
Subject: Re: Difficult molecular replacement in R32 with pseudo translation
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-10-19


Subject: Difficult molecular replacement in R32 with pseudo translation
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2009-10-19


Dear All:

I got a problem with a data set in R32 space group with a resolution of 2.6.
Pointless indicated that the space group was R32 and phenix triage showed that there was a pseudotranslation in the ASU.

My protein is a hexamer and the matthew coeff. showed that there should just be two monomers in one ASU of R32.

However, phaser in R32 and Molrep in R32 gave different solutions because Molrep used the pseudo-translation. I am not sure which one was correct because refinement of both solutions get stuck around 35% for Rfactor.

I tried the molecular replacement in P1 and I got good solutions in Phaser. I think they are correct solutions because my protein was a hexamer and the crystal packing in P1 was formed by hexamers.

How can I get correct solutions in R32 and finish the refinement?

Many thanks in advance.

Jerry McCully







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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: how to improve Rfree?
From: Bart Hazes Bart {- dot -} Hazes {- at -} UALBERTA {- dot -} CA
Date: 2009-10-19		Next message:
Subject: Re: Difficult molecular replacement in R32 with pseudo translation
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-10-19
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