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Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

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CCP4bb <-- 2007 <-- June 2007 <-- 08 June 2007

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From: Anthony Addlagatta anthony {- at -} UOXRAY {- dot -} UOREGON {- dot -} EDU
Date: 2007-06-08

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From: "Bryan W {- dot -} Lepore" bryanlepore {- at -} MAIL {- dot -} UTEXAS {- dot -} EDU
Date: 2007-06-08

Subject:
From: "Demetres D {- dot -} Leonidas" ddl {- at -} EIE {- dot -} GR
Date: 2007-06-08

Dear Sajid,

the problem with all oils is that they allow some small evaporation that
is why your drops dry out. You can set up your crystal drops over a
reservoir (you use microbridges) which will be the same as your
crystallization media and your drops won't dried out.

best of luck

Demetres

sajid akthar wrote:
> Hi All
>
> I'm getting clusters of crystals (tiny and long needles) and I'm trying to get good single crystals. Even I'm getting the same result at low salt concentrations. I reduced the temperature to slow down the evaporation, still no change. So I'm trying with mineral oil. Here I'm getting somewhat thicker needles and the number of needles is reduced. But my drop seems to be dried out in two or three days (I dont think there is a sealing problem). Can anyone suggest me to overcome this and to get good crystals.
>
> Thankz in advance
>
> Sajid
>
>
> Download prohibited? No problem! To chat from any browser without download, Click Here: http://in.messenger.yahoo.com/webmessengerpromo.php
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>

--
Demetres D. Leonidas, Ph.D.
Structural Biology & Chemistry Group
Institute of Organic and Pharmaceutical Chemistry
The National Hellenic Research Foundation
48, Vassileos Constantinou Avenue
Athens 116 35, Greece
==================================================
Tel. +30 210 7273841 (office)
+30 210 7273895 (lab)
Fax. +30 210 7273831
E-mail: ddl@eie.gr
URL: http://athena.eie.gr
==================================================

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CCP4bb <-- 2007 <-- June 2007 <-- 08 June 2007

Previous message:
Subject:
From: Anthony Addlagatta anthony {- at -} UOXRAY {- dot -} UOREGON {- dot -} EDU
Date: 2007-06-08

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From: "Bryan W {- dot -} Lepore" bryanlepore {- at -} MAIL {- dot -} UTEXAS {- dot -} EDU
Date: 2007-06-08