Re: [ccp4bb] SAD phasing at home source

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inclusion bodies centrifugation
From: Ezra Peisach epeisach {- at -} GMAIL {- dot -} COM
Date: 2009-10-28

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Subject: Re: Inclusion bodies centrifugation
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2009-10-28

Subject: Re: SAD phasing at home source
From: Flip Hoedemaeker flip {- at -} FORMULATRIX {- dot -} COM
Date: 2009-10-28

Yep, solved a structure at Cu K-alpha once with the sulfur signal,
resolution 1.7A and a redundancy of 50 or so did the trick. Actually,
one of the sulphurs we found was an ordered SO4 molecule, so if you have
that in your crystallisation condition it might be a bonus...

Flip

Watanabe Nobuhisa wrote:
> If your crystal diffracts well and tough enough for high redundant data
> collection, no HA or using sulfur in your protein will be the first
> choice. Even at Cu K-alpha. I think...
>
> N. Watanabe
>
> On 2009/10/27, at 3:32, james09 pruza wrote:
>
>> Dear All,
>>
>> I need some suggestions regarding the SAD phasing using home source.
>> What the the most commonly used HA for SAD phasing for Cu anode.
>> All suggestions are welcome.
>> Thanks.
>>
>> James
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inclusion bodies centrifugation
From: Ezra Peisach epeisach {- at -} GMAIL {- dot -} COM
Date: 2009-10-28

Next message:
Subject: Re: Inclusion bodies centrifugation
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2009-10-28