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Re: [ccp4bb] Crystallization of lysine and arginine rich proteins
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Crystallization of lysine and arginine rich proteins
From: Jan Rash janrsh {- at -} GOOGLEMAIL {- dot -} COM
Date: 2009-11-02		Next message:
Subject: Re: Crystallization of lysine and arginine rich proteins
From: Flip Hoedemaeker flip {- at -} FORMULATRIX {- dot -} COM
Date: 2009-11-02


Subject: Re: Crystallization of lysine and arginine rich proteins
From: Stephen Graham scg34 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2009-11-02

Try reductive methylation of the lysines:
Walter, T. S., Meier, C., Assenberg, R., Au, K. F., Ren, J., Verma,
A., Nettleship, J. E., Owens, R. J., Stuart, D. I. & Grimes, J. M.
(2006). Structure, 14, 1617–1622.

Cheers,

Stephen

2009/11/2 Jan Rash :
> Dear All,
>
>  I have a question regarding the crystallization of lysine and arginine rich
> protein around 13%. So far our attempts to crystallize this protein have not
> been successful although the secondary structure predictions, CD
> spectroscopy measurements clearly show that this protein is folded. I
> presume that these lysine and arginine are the sources of the local
> flexibility in the protein even though the protein is globular overall.
> Moreover, my attempts to crystallize the limited proteolysis fragments also
> did not achieve crystals. I have also tried the crystallization with its
> binding partners and could not succeed.  I think any compound that binds to
> the lysine/arginine side chains might affect the crystallization process
> thereby reducing the internal flexibility of protein.  Can anybody suggest
> some effective strategy for the crystallization?
>
>
>
> Thanks
>
> Umar
>
>



--
Dr Stephen Graham
1851 Research Fellow
Cambridge Institute for Medical Research
Wellcome Trust/MRC Building
Addenbrooke's Hospital, Hills Road
Cambridge, CB2 0XY, UK
Phone: +44 1223 762 638
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