Re: [ccp4bb] Crystallization of lysine and arginine rich proteins

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Crystallization of lysine and arginine rich proteins
From: Stephen Graham scg34 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2009-11-02

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Subject: Re: Crystallization of lysine and arginine rich proteins
From: Simon Bushell srbushellbbs {- at -} GOOGLEMAIL {- dot -} COM
Date: 2009-11-02

Subject: Re: Crystallization of lysine and arginine rich proteins
From: Flip Hoedemaeker flip {- at -} FORMULATRIX {- dot -} COM
Date: 2009-11-02

Hi Jan,

We have tried crystallizing a similar protein without success, later on
it turned out that the protein was having a strong interaction with DNA
that was not sequence specific. You might have a case like that....

Flip

Jan Rash wrote:
> Dear All,
>
> I have a question regarding the crystallization of lysine and arginine
> rich protein around 13%. So far our attempts to crystallize this protein
> have not been successful although the secondary structure predictions,
> CD spectroscopy measurements clearly show that this protein is folded. I
> presume that these lysine and arginine are the sources of the local
> flexibility in the protein even though the protein is globular overall.
> Moreover, my attempts to crystallize the limited proteolysis fragments
> also did not achieve crystals. I have also tried the crystallization
> with its binding partners and could not succeed. I think any compound
> that binds to the lysine/arginine side chains might affect the
> crystallization process thereby reducing the internal flexibility of
> protein. Can anybody suggest some effective strategy for the
> crystallization?
>
>
>
> Thanks
>
> Umar
>
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallization of lysine and arginine rich proteins
From: Stephen Graham scg34 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2009-11-02

Next message:
Subject: Re: Crystallization of lysine and arginine rich proteins
From: Simon Bushell srbushellbbs {- at -} GOOGLEMAIL {- dot -} COM
Date: 2009-11-02