Re: [ccp4bb] Crystallization of lysine and arginine rich proteins

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallization of lysine and arginine rich proteins
From: Andy Torelli att29 {- at -} CORNELL {- dot -} EDU
Date: 2009-11-02

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From: "Oganesyan, Vaheh" OganesyanV {- at -} MEDIMMUNE {- dot -} COM
Date: 2009-11-02

Subject: Re: Crystallization of lysine and arginine rich proteins
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2009-11-02

Before deciding to change the protein sequence it is best to look at the
precipitation pattern of the protein as a function of various precipitants.
If this shows that precipitation is not within the standard range commonly
observed for other basic proteins even at high protein concentrations
then side chain mutation should be tried.
Even lysozyme would be hard to crystallize at 1 mg/ml at 38°C.

E.
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CEA Saclay, 91191 Gif-sur-Yvette
Cedex FRANCE http://www-dsv.cea.fr/en/ibitecs/82
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: estura@cea.fr Fax: 33 (0)1 69 08 90 71

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallization of lysine and arginine rich proteins
From: Andy Torelli att29 {- at -} CORNELL {- dot -} EDU
Date: 2009-11-02

Next message:
Subject: definition file
From: "Oganesyan, Vaheh" OganesyanV {- at -} MEDIMMUNE {- dot -} COM
Date: 2009-11-02