Re: [ccp4bb] sulfur sad phasing

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: sulfur sad phasing
From: Matthew BOWLER bowler {- at -} ESRF {- dot -} FR
Date: 2009-11-11

Next message:
Subject: Re: sulfur sad phasing
From: Miguel Ortiz Lombardia miguel {- dot -} ortiz-lombardia {- at -} AFMB {- dot -} UNIV-MRS {- dot -} FR
Date: 2009-11-11

Subject: Re: sulfur sad phasing
From: Vellieux Frederic Frederic {- dot -} Vellieux {- at -} IBS {- dot -} FR
Date: 2009-11-11

Matthias Zebisch wrote:
> Dear bb!
>
> What is the optimal wavelength for Sulfur SAD phasing?
> Is it 1.9A or should one go below that to reduce absorption/damage.
>
> Also, would the same wavelength be appropriate to maximize anomalous
> scattering to position chlorides, calcium, sulfate in already phased
> structures?
>
> Thanks in advance,
>
> Matthias
>
>
Hi,

The Sulphur absorption edges are way too far for realistic data
collection (can't remember the precise values right now).

But Sulphur SAD can even be done on a home source (Cu K-alpha), see eg
http://www.stoe.com/pages/brochure/Genix%20Cu%20labnote.pdf

there the precision of measurements is very important.

Fred.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: sulfur sad phasing
From: Matthew BOWLER bowler {- at -} ESRF {- dot -} FR
Date: 2009-11-11

Next message:
Subject: Re: sulfur sad phasing
From: Miguel Ortiz Lombardia miguel {- dot -} ortiz-lombardia {- at -} AFMB {- dot -} UNIV-MRS {- dot -} FR
Date: 2009-11-11