Re: [ccp4bb] trouble with protein storage

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Subject: Re: trouble with protein storage
From: Annie Hassell annie {- dot -} m {- dot -} hassell {- at -} GSK {- dot -} COM
Date: 2009-11-19

Marie-Helene--

We have included 3-5% 1,2-propanediol in the protein buffer (as a
substitute for glycerol) to stabilize proteins and have seen improvement
in the long term storage in many cases.

Hope this helps!
annie

Annie Hassell
Glaxo Smithkline
5 Moore Drive
RTP, NC 27709
919/483-3228
919/483-0368 (FAX)
annie.m.hassell@gsk.com

"LEDU Marie-Helene 161111"
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[ccp4bb] trouble with protein storage

Hello,

Sorry for the unrelated question.
We encounter important storage problem of our favorite protein.
We can not freeze it without a lost of 50 to 90 %, and can not use
glycerol without precipitation.
Would anybody have suggestions or references that could help us ?
Thanks a lot in advance.

Marie-Helene

Dr Marie-Hélène LeDu
CEA/DSV/IBITEC-S/LBSR
bat 144, CE Saclay
91191 Gif-sur-Yvette
FRANCE
tel 01 69 08 71 35
emel : marie-helene.ledu@cea.fr

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Stereo 3D notebook
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2009-11-19