- Protein crystallography
Main steps: - Protein purification - Crystallisation
Special: - Programs for crystallography - X-ray detectors
Basic tutorials: - Chemistry - Protein - Peptide - Amino Acids
Xtal community: - CCP4BB
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Subject: hairpin formation in gene From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM Date: 2009-11-20
Dear bb-ers,
I am trying to have a gene synthesized and found out that it forms an 11-bp hairpin. Does that complicate expression? Would it be better to try and disrupt it by altering codon usage to improve expression?
Thank you in advance,
Sangeetha.
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