Re: [ccp4bb] B-factor sharpening in CCP4 or Coot

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: automatic hydrophobic core (buried residues) determination
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2007-06-14

Next message:
Subject: Problem with ligand refinement in REFMAC5
From: Andreas Hörnberg andreas {- dot -} hornberg {- at -} UCMP {- dot -} UMU {- dot -} SE
Date: 2007-06-14

Subject: Re: B-factor sharpening in CCP4 or Coot
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-06-14

There is a CAD option to apply a scale and B factor to any set of data,
and an FFT option to apply a scale and B factor to any column in the map.
Check the documentation for whether you need SCALE 1 -10.0 or SCALE 1
+10.0 !

Eleanor

Jeff Lee wrote:
> Hi,
>
> I have a question for everyone. I wanted to up-weight my high
> resolution terms in my electron density map by applying a B-factor
> sharpening term. I have mtz files produced from DM and I usually use
> Coot to generate my electron density maps. I was wondering is there
> an easy way to apply a B-factor sharpening term in Coot? If not, is
> there a program in ccp4 where by I can set the B-sharp factor?
>
> Thanks
>
> Jeff
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Problem with ligand refinement in REFMAC5
From: Andreas Hörnberg andreas {- dot -} hornberg {- at -} UCMP {- dot -} UMU {- dot -} SE
Date: 2007-06-14