Re: [ccp4bb] SelenoMet protein?

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SelenoMet protein?
From: "W {- dot -} M {- dot -} B {- dot -} " butianyuri {- at -} GMAIL {- dot -} COM
Date: 2007-06-14

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Subject: bond length constraints in refmac
From: Craig McElroy mcelroy {- dot -} 31 {- at -} OSU {- dot -} EDU
Date: 2007-06-14

Subject: Re: SelenoMet protein?
From: jjwarren {- at -} DUKE {- dot -} EDU jjwarren {- at -} DUKE {- dot -} EDU
Date: 2007-06-14

2 methionines in 63 amino acids might work. However, if I'm reading your
message correctly, the methionines are likely to be located in disordered
loops, which makes them less of a good bet.

One alternative to consider is using modified DNA oligos, e.g. with
5-iodo-deoxyuracil or 5-bromo-deoxyuracil substituted for thymine or with
5-iodo or bromo cytosine. These are pretty widely available (e.g. from
www.oligos.com), and not particularly expensive. You can use them to solve by
SAD/SIRAS or MAD in the case of Br derivitives.

Quoting "W.M. B." :

> Hi All
>
> I have a protein-DNA complex dataset, and can't solve it by MR. I would like
> to hear your advise whether I should prepare the selenomet protein. The
> native protein is homeodomain protein, which has 63 AA. It only has two Met
> in the cloning artifacts. There are two complexes in the AU. Will MAD be
> working? Did anyone have similat experience?
>
> Thanks for your response.
>
> Best
>
> Steve
>

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SelenoMet protein?
From: "W {- dot -} M {- dot -} B {- dot -} " butianyuri {- at -} GMAIL {- dot -} COM
Date: 2007-06-14

Next message:
Subject: bond length constraints in refmac
From: Craig McElroy mcelroy {- dot -} 31 {- at -} OSU {- dot -} EDU
Date: 2007-06-14