[ccp4bb] Solubilization buffer

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refining residues as rigid bodies
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-12-02

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Subject: Re: Solubilization buffer
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2009-12-02

Subject: Solubilization buffer
From: megha goyal mgbiotec {- at -} GMAIL {- dot -} COM
Date: 2009-12-02

Hi all,

We use 8M urea solubilization buffer for our protein in inclusion bodies and
recommended temperature is 10-15º C. but in 8M conc the urea does not
dissolve and is in crystalline form only, will it have any effect on
solubilzation efficiency. Our solubilization time is 1 Hr and after that we
centrifuge and use the supernatant for refolding via dialysis. however the
pellet after centrifugation of solubilzation show presence of our protein on
sds page analysis. what should we do so that the process of solubilization
is complete and our protein is not lost in pellet.

thanks in anticipation.

meg

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refining residues as rigid bodies
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-12-02

Next message:
Subject: Re: Solubilization buffer
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2009-12-02