[ccp4bb] UV detection of crystals/survey

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Solubilization buffer
From: Joerg Standfuss jst {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2009-12-03

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Subject: Re: Solubilization buffer
From: Savvas Savvides savvas {- dot -} savvides {- at -} UGENT {- dot -} BE
Date: 2009-12-03

Subject: UV detection of crystals/survey
From: tommi kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2009-12-03

Hello,

I would be interested in people experiences and opinions about what
types of fluorescence microscope setups have worked
adequately to distinguish small protein crystals from background/
autofluorescence etc. ...general comments?
(regular dichroic mirros etc dont allow Trp peak to pass through for
instance etc and quartz becomes qute expensive i think?)
i am looking for the cheapest but effective system (ie i want to see
10 um size things)

regular sterefluor. microscope with filters + fiber UV light from the
side? anybody has found something like that to work?

thank you very much for comments,
Tommi

Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Solubilization buffer
From: Joerg Standfuss jst {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2009-12-03

Next message:
Subject: Re: Solubilization buffer
From: Savvas Savvides savvas {- dot -} savvides {- at -} UGENT {- dot -} BE
Date: 2009-12-03