[ccp4bb] How to reduce free-R factor

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-12-03

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Subject: Re: How to reduce free-R factor
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-12-04

Subject: How to reduce free-R factor
From: Pankaj Chauhan pankajimtech {- at -} GMAIL {- dot -} COM
Date: 2009-12-03

Hi all,

In one of my 2.52 A structure, which is dimeric, the R-factor is 21.4 and
free-R is 29.3. I have tried all ways to reduce the free-R including TLS
(even by defining the number of TLS group per chain), but still factors are
not getting reduced. Even i have tried some other space group, but no use.
Even i have the same structure with some complex at 1.86 A in the same space
group , but everything is fine in that.
Any suggestion,
Regards,

--
Pankaj Chauhan
Senior Research Fellow, Graduate student
Crystallography Laboratory
Division of Protein Science & Engg.
IMTECH , Chandigarh, INDIA

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: How to add a single water molecule
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-12-03

Next message:
Subject: Re: How to reduce free-R factor
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2009-12-04