Re: [ccp4bb] where I have been going wrong in crystallization?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: where I have been going wrong in crystallization?
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2009-12-21

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Subject: Re: where I have been going wrong in crystallization?
From: mjvdwoerd {- at -} NETSCAPE {- dot -} NET mjvdwoerd {- at -} NETSCAPE {- dot -} NET
Date: 2009-12-21

Subject: Re: where I have been going wrong in crystallization?
From: David Briggs drdavidcbriggs {- at -} GMAIL {- dot -} COM
Date: 2009-12-21

Hi Martyn,

this recipe tends to work for me...

Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8
Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8
Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88

Mix equal amounts of lysozyme with reagent, incubate at 4 or 22
degrees Celsius. Batch or vapor diffusion works fine.

==> Direct copy/paste from http://hamptonresearch.com/experiments.aspx <==

HTH and Happy Christmas.

Cheers,

Dave

============================
David C. Briggs PhD
============================
University of Manchester E-mail:
david.c.briggs@manchester.ac.uk
============================
Twitter: @xtaldave
Skype: DocDCB
============================

2009/12/21 MARTYN SYMMONS :
> Dear All
> checking out the Lysozyme crystallization methods on the web I liked the Rigaku Instructions that I found:
> (http://www.rigaku.com/protein/crystallization.html)
>
> "...create a drop of 3ul lysozyme solution, and 3 ul of well solution, respectfully, for a total drop size of 6ul..."
>
> So perhaps sometimes I am just not respectful enough to deserve crystals ?
>
> good wishes to all
> regards,
> Martyn
> -----------------------
> Martyn Symmons
> MRC-MBU Cambridge UK
> 'Chan fhiosrach mur feòraich.'
> Gaelic proverb -
> Nothing asked, nothing learned.
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: where I have been going wrong in crystallization?
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2009-12-21

Next message:
Subject: Re: where I have been going wrong in crystallization?
From: mjvdwoerd {- at -} NETSCAPE {- dot -} NET mjvdwoerd {- at -} NETSCAPE {- dot -} NET
Date: 2009-12-21