Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

[ccp4bb] Lots of noise in structure

- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Anions in protein structures
From: "Clayton, Gina Martyn" gina_clayton {- at -} MERCK {- dot -} COM
Date: 2010-01-05
Next message:
Subject: Re: Judging a homology model
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2010-01-06


Subject: Lots of noise in structure
From: Rajan rajanvyas {- at -} GMAIL {- dot -} COM
Date: 2010-01-06

Dear All,

Happy New Year

I am working with a 2.06 Ang resolution structure in F432 space group and
further using REFMAC (also tried Phenix) program for refinement of my
model.

Every thing in the structure seems good, R factor/ R free= 18.59/20.77 but
the structure is too noisy (blobs of difference Fourier with red as well as
green density).

As such electron density is looking good for the protein part.

Is there any parameter which i had to check.

Thanking you in advance

Rajan

--
************************************************
Current Address:

Rajan Vyas
Research Scholar
Deptt. of Biotechnology
Panjab University Chandigarh, India
160014 Mob. +919417374197
Fax: +91-172-2625254
************************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Anions in protein structures
From: "Clayton, Gina Martyn" gina_clayton {- at -} MERCK {- dot -} COM
Date: 2010-01-05
Next message:
Subject: Re: Judging a homology model
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2010-01-06



ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd