Re: [ccp4bb] Lots of noise in structure

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

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- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Judging a homology model
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2010-01-06

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Subject: Re: Lots of noise in structure
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Date: 2010-01-06

Subject: Re: Lots of noise in structure
From: Gerard DVD Kleywegt gerard {- at -} XRAY {- dot -} BMC {- dot -} UU {- dot -} SE
Date: 2010-01-06

> Is there any parameter which i had to check.

Yes, the contour levels of your mFo-DFc map...

(My guess is that you are contouring at an arbitrary low level - say, "3
sigma".)

--dvd

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Gerard J. Kleywegt
Dept. of Cell & Molecular Biology University of Uppsala
Biomedical Centre Box 596
SE-751 24 Uppsala SWEDEN

http://xray.bmc.uu.se/gerard/ mailto:gerard@xray.bmc.uu.se
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Judging a homology model
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2010-01-06

Next message:
Subject: Re: Lots of noise in structure
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-01-06