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Re: [ccp4bb] Low resolution chain tracing |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Low resolution chain tracing From: "Fan, Hai-fu" fanhf {- at -} CRYST {- dot -} IPHY {- dot -} AC {- dot -} CN Date: 2007-06-18 Dear Alexei, I would suggest an alternative way to try. We have an example similar to your case. A set of 3.3Angstrom resolution Se-SAD data phased by SOLVE/RESOLVE led to a map showing clearly the secondary structure but with poor evidence on side chains. A model of poly-alanine fragments was than built manually. While the sigmaA weighted Fourier map based on this model could not improve the map, a single run of OASIS-DM revealed some side chains. A portion of the map are attached, in which a - SOLVE/RESOLVE map with the poly-alanine model superimposed, b - SOLVE/RESOLVE map with the final model superimposed, c - SigmaA map with the final model superimposed, d - OASIS-DM map with the final model superimposed. If you need further details, please contact Dr. Yao, Deqiang at dqyao@cryst.iphy.ac.cn Hai-fu ---------------------------- Professor Fan, Hai-fu Institute of Physics Chinese Academy of Sciences Beijing 100080, P.R. China URL: http://cryst.iphy.ac.cn CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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