Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

Re: [ccp4bb] Low resolution chain tracing

- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: conversion of .fcf to 2fo-fc.map and fo-fc.map of ccp4 format
From: deliang chen deliangc {- at -} UCI {- dot -} EDU
Date: 2007-06-18
Next message:
Subject: libstdc++.so.5: cannot open shared object file: No such file or directory
From: Paul Kraft haresear68 {- at -} YAHOO {- dot -} COM
Date: 2007-06-18


Subject: Re: Low resolution chain tracing
From: "Fan, Hai-fu" fanhf {- at -} CRYST {- dot -} IPHY {- dot -} AC {- dot -} CN
Date: 2007-06-18

Dear Alexei,

I would suggest an alternative way to try. We have an example similar to
your case. A set of 3.3Angstrom resolution Se-SAD data phased by
SOLVE/RESOLVE led to a map showing clearly the secondary structure but with
poor evidence on side chains. A model of poly-alanine fragments was than
built manually. While the sigmaA weighted Fourier map based on this model
could not improve the map, a single run of OASIS-DM revealed some side
chains. A portion of the map are attached, in which

a - SOLVE/RESOLVE map with the poly-alanine model superimposed,
b - SOLVE/RESOLVE map with the final model superimposed,
c - SigmaA map with the final model superimposed,
d - OASIS-DM map with the final model superimposed.

If you need further details, please contact Dr. Yao, Deqiang at
dqyao@cryst.iphy.ac.cn

Hai-fu

----------------------------

Professor Fan, Hai-fu
Institute of Physics
Chinese Academy of Sciences
Beijing 100080, P.R. China

URL: http://cryst.iphy.ac.cn











ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd