Re: [ccp4bb] Blob help

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Blob help
From: Vin Purp buckybadger97 {- at -} NETSCAPE {- dot -} NET
Date: 2010-01-08

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Subject: Re: Reg. Protein purification
From: Quyen Hoang qqhoang {- at -} GMAIL {- dot -} COM
Date: 2010-01-08

Subject: Re: Blob help
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-01-08

Hello Vin,

have you tried fitting two glycerol molecules with complementary
occupancies?

Tim

--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A

On Fri, 8 Jan 2010, Vin Purp wrote:

> Can a trained eye identify this blob? This is 1.65 Ang resolution. There are
> very few components in the M.L. and protein purification buffers:
>
> ~1.5 ammonium sulfate, ~50 mM TRIS-HCl buffer pH 8.5, ~5 mM
> 2-mercaptoethanol, and ~25% glycerol. That's it.
>
>
> Glycerol fits the 'forked' portion, but does not account for the opposite
> end that looks like a carboxylate. Are there any common contaminants
> associated with glycerol (from aging), or left overs from synthesis (dirty
> batch)? Any help is appreciated.
>
> V
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Blob help
From: Vin Purp buckybadger97 {- at -} NETSCAPE {- dot -} NET
Date: 2010-01-08

Next message:
Subject: Re: Reg. Protein purification
From: Quyen Hoang qqhoang {- at -} GMAIL {- dot -} COM
Date: 2010-01-08