Re: [ccp4bb] question on the effect of a fire alarm on crystallization

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: question on the effect of a fire alarm on crystallization
From: Djordje Francuski francuz {- at -} CHEMIE {- dot -} FU-BERLIN {- dot -} DE
Date: 2010-01-22

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Subject: Re: question on the effect of a fire alarm on crystallization
From: Sean Seaver sean {- at -} P212121 {- dot -} COM
Date: 2010-01-22

Subject: Re: question on the effect of a fire alarm on crystallization
From: Ganesh Natrajan natrajan {- at -} EMBL {- dot -} FR
Date: 2010-01-22

Hi ,

Maybe the lower frequency of the earthquake vibrations or the
handling helped your crystals nucleate. I would assume that even the
resonance frequency of the linbro plates are low, and may cause
disturbances in them. However, fire alarm systems usually are of a much
higher frequency (shrill whines and stuff ), and so they may not affect the
linbro plates.

Ganesh

On Fri, 22 Jan 2010 17:08:41 +0100,
Herman.Schreuder@SANOFI-AVENTIS.COM wrote: When working on tobacco Rubisco
in Los Angeles, we got the best crystals of the activated form of the
protein after small earthquakes (4-5 on the richter scale). However, as
people in the lab. noticed, this earthquake parameter is somewhat difficult
to control. Handling the linbro plates sometimes caused massive nucleation:
I would look at a plate with only clear drops and by the time I would put
the plate back in the incubator there was so much nucleation that the plate
was of no use any more. When the nucleation of your protein is sensitive to
vibrations, I would worry more about handling than about the fire alarm.
Best, Herman

-------------------------
FROM: CCP4 bulletin board
[mailto:CCP4BB@JISCMAIL.AC.UK] ON BEHALF OF Jacqueline Vitali
SENT: Friday,
January 22, 2010 3:14 PM
TO: CCP4BB@JISCMAIL.AC.UK
SUBJECT: [ccp4bb]
question on the effect of a fire alarm on crystallization

Dear all,
Does anyone know the effect of a fire alarm placed in the same room
where one grows crystals? (1) On trays placed inside styrofoam boxes
(2) On trays placed inside an incubator which has not been tested as
vibration free. Thank you. Jackie Vitali

--
What is true
for E. coli is also true for an elephant - Jacques Monod.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: question on the effect of a fire alarm on crystallization
From: Djordje Francuski francuz {- at -} CHEMIE {- dot -} FU-BERLIN {- dot -} DE
Date: 2010-01-22

Next message:
Subject: Re: question on the effect of a fire alarm on crystallization
From: Sean Seaver sean {- at -} P212121 {- dot -} COM
Date: 2010-01-22