Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Very high B-factors of some atoms of ligand in Refmac
From: Clemens Vonrhein vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-01-28

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Subject: Re: Very high B-factors of some atoms of ligand in Refmac
From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM
Date: 2010-01-28

Subject: Re: Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM
Date: 2010-01-28

Thanks for your response, Edward. Yes, that is what I thought. For the most
part, people plan to tag their proteins in my lab. I'll possibly be the only
one purifying non-tagged proteins in my lab. I love the AKTA. Another was
all praise of the Profinia. To my knowledge, tagged proteins don't always
get fully purified with just an affinity column. An additional size
exclusion step helps. That is, if the tag is even available at the surface
for binding the affinity column. My vote is AKTA. So, I wanted to find out
what the world thought about both.

On Thu, Jan 28, 2010 at 11:16 AM, Edward Collins > wrote:

> personally, I don't know why you would want to limit yourself to just using
> affinity tags to purify your proteins. For my money the flexibility of the
> AKTA is critical.
> good luck
> -ed
>
> On Jan 28, 2010, at 11:08 AM, Sangeetha Vedula wrote:
>
> > Dear all,
> >
> > My lab is looking into buying a protein purification system. The AKTA is
> more versatile, but does anyone know if the Profinia is in any way superior
> to the AKTA for purification of affinity-tagged proteins? If it is, I would
> really appreciate if you can tell me in what way it is superior.
> >
> > Thanks,
> >
> > Sangeetha.
>
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Very high B-factors of some atoms of ligand in Refmac
From: Clemens Vonrhein vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-01-28

Next message:
Subject: Re: Very high B-factors of some atoms of ligand in Refmac
From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM
Date: 2010-01-28