| Quick navigation: | Home | Site Map || References | Biography || Copyright | Other copyright | Contact us | Advert | | |||
Re: [ccp4bb] Vapor diffusion calculator |
||||
- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Vapor diffusion calculator From: Clemens Grimm Clemens {- dot -} Grimm {- at -} BIOZENTRUM {- dot -} UNI-WUERZBURG {- dot -} DE Date: 2010-02-03 Zitat von Imre Toeroe > Hi, > > this might be relevant here: > > http://scripts.iucr.org/cgi-bin/paper?S0907444995000436 > Yes! thanks for the link. > Imre > > Clemens Grimm wrote: >> regardless of what such a (kinetic) calculator will calculate at the end - >> we will have to feed it with data of the type "vapour pressure vs. >> concentration and temperature" of substance X. Even without a whole >> simulation tool for the kinetics of a vapour diffusion experiment >> this will already enable us to tell what the equilibrium >> state/endpoint of the experiment will be. >> As the substances we are interested in are typically stuff like >> PEGs and at very high concentration (non-ideal behaviour!), we will >> need experimental data. For some salts there are tables. But for >> PEGs etc. this has to be generated. I feel that only having this >> kind of data at hand would already be tremendously useful! >> >> Clemens >> >> >> Zitat von Jacob Keller >> >> Dear Crystallographers, >> >> Is anybody aware of a calculator for vapor diffusion experiments to >> plot concentrations of various solvent components as a function of >> time? For a simple example, what happens when I mix a protein >> solution containing 50mM NaCl 1:1 with a reservoir containing 50% >> MPD but no salt? Where is the vapor diffusion equilibrium, and how >> does the drop composition change as a function of time? More >> complicated would be experiments involving volatile components >> other than water, as I think, for example, ethanol would almost >> instantly equilibrate, then the water diffusion would kick in over >> a longer time scale. Even more complicated would be pH-dependent >> volatilities such as acetate. I don't think this would be >> impossible to figure out, but it would be nice if there were a >> pre-existing tool/server to do such. >> >> Regards, >> >> Jacob Keller >> >> >> ******************************************* >> Jacob Pearson Keller >> Northwestern University >> Medical Scientist Training Program >> Dallos Laboratory >> F. Searle 1-240 >> 2240 Campus Drive >> Evanston IL 60208 >> lab: 847.491.2438 >> cel: 773.608.9185 >> email: j-keller2@northwestern.edu >> ******************************************* >> >> >> -------------------------------------------------- >> Dr. Clemens Grimm >> Institut für Biochemie >> Biozentrum der Universität Würzburg >> Am Hubland >> D-97074 Würzburg >> Germany >> e-mail: Clemens.Grimm@biozentrum.uni-wuerzburg.de >> phone : +49 0931 888 84031 >> ------------------------------------------------- > > -------------------------------------------------- Dr. Clemens Grimm Institut für Biochemie Biozentrum der Universität Würzburg Am Hubland D-97074 Würzburg Germany e-mail: Clemens.Grimm@biozentrum.uni-wuerzburg.de phone : +49 0931 888 84031 ------------------------------------------------- CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
|||
| ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd |