[ccp4bb] SOLVE help

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: how to improve resolution
From: Annie Hassell annie {- dot -} m {- dot -} hassell {- at -} GSK {- dot -} COM
Date: 2010-02-05

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Subject: SOLVE help
From: Ankit Gupta ankitgpta {- at -} GMAIL {- dot -} COM
Date: 2010-02-05

Subject: SOLVE help
From: Ankit Gupta ankitgpta {- at -} GMAIL {- dot -} COM
Date: 2010-02-05

Hello,

I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD
dataset (inflexion and remote : 3 A). The SAD dataset when searched for
heavy atom sites with a native dataset (SIRAS) gives a figure of merit of
0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of
merit (~0.18).

My question is :

How can I use the good sites from the SAD dataset for their refinement using
SOLVE iwith the MAD dataset? There is a keyword in SOLVE named 'REFINEALL'
but I am not sure if it helping me. If I try to use the keyword, along with
SCALE_MAD and ANALYZE_SOLVE, it reduces the number of sites and gives a low
figure of merit.

Any suggestions on this will be appreciated.

With Regards,
Ankit

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: how to improve resolution
From: Annie Hassell annie {- dot -} m {- dot -} hassell {- at -} GSK {- dot -} COM
Date: 2010-02-05

Next message:
Subject: SOLVE help
From: Ankit Gupta ankitgpta {- at -} GMAIL {- dot -} COM
Date: 2010-02-05