Re: [ccp4bb] how to improve resolution

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: reforigin on 2FKA
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-02-05

Next message:
Subject: Re: how to improve resolution
From: Bernhard Rupp br {- at -} RUPPWEB {- dot -} ORG
Date: 2010-02-05

Subject: Re: how to improve resolution
From: Ho Leung Ng ho {- at -} CONFOMETRX {- dot -} COM
Date: 2010-02-05

My interpretation of hollow crystals is that the crystals are growing
too fast. I've had success with similar looking crystals by slowing
crystal growth. You can try lower temperature, protein concentration,
or precipitant. In my case, I found success by trying different
concentrations of NaCl in the reservoir (see Janet Newman, Acta Cryst
D, 61:490).

ho

From: rui
Subject: how to improve resolution

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Hi, All,

We are trying to crystallize a protein and found some initial hit in the
following conditions,

pH 4.8, 0.2 M AS or some other salts ( NaCl,LiCl, MgCl2 ), 32% PEG4000 or
PEG3350 ). However the quality of the crystal is not so great,some of them
look like needle cluster(very long in length), some of them look like
multi-crystals or hollow inside. We tried to optimize the pH and PEG and
tested one that diffracts at 2.9A. For the next, how to improve
resolution?Any suggestions? Even mutate the protein to get a high resolution
is ok, generally what kind of mutation would make proteins crystallize
better? Thanks.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: reforigin on 2FKA
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-02-05

Next message:
Subject: Re: how to improve resolution
From: Bernhard Rupp br {- at -} RUPPWEB {- dot -} ORG
Date: 2010-02-05