[ccp4bb] How to replace 2.45M K/Na phosphate buffer

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: shi jiahai jiahai {- dot -} shi {- at -} GMAIL {- dot -} COM
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Subject: How to replace 2.45M K/Na phosphate buffer - 2
From: Claudia Scotti claudiascotti {- at -} HOTMAIL {- dot -} COM
Date: 2007-06-22

Subject: How to replace 2.45M K/Na phosphate buffer
From: Claudia Scotti claudiascotti {- at -} HOTMAIL {- dot -} COM
Date: 2007-06-22

Dear list,

I've crystals in a condition including 2,45 M K/Na phosphate buffer for the well condition, and 1 microl protein + 0.8 microl Na/K phosphate buffer + 0.25 microl 30% 1,6-hexanediol in the drop condition.

These crystals dissolve in several cryoprotectant solutions and survive only in malonic acid, with which they give diffraction at 7-8 A.

Among the several different possibilities, I'd like to try to optimize the crystals, and one possiblity among others would be to change the buffer.

Any suggestions, please, about how to replace K/Na phosphate? At these concentrations it is acting as a buffer but also as a precipitant and I was wondering if anybody has experienced nice buffer-salt combinations that proved to be useful starting from a similar condition.

Thanks a lot,

Claudia

Claudia Scotti
Dipartimento di Medicina Sperimentale
Sezione di Patologia Generale
Universita' di Pavia
Piazza Botta, 10
27100 Pavia
Italia
Tel. 0039 0382 986335/8/1
Facs 0039 0382 303673
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Save image in Postscript format from PYMOL
From: shi jiahai jiahai {- dot -} shi {- at -} GMAIL {- dot -} COM
Date: 2007-06-22

Next message:
Subject: How to replace 2.45M K/Na phosphate buffer - 2
From: Claudia Scotti claudiascotti {- at -} HOTMAIL {- dot -} COM
Date: 2007-06-22