[ccp4bb] PEG interaction with Trp in active site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: phi/psi angle display in coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2010-02-19

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Subject: Re: anomalous difference fourier maps
From: Gerard Bricogne gb10 {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-02-19

Subject: PEG interaction with Trp in active site
From: Marek Frischerkase frischerkasemarek {- at -} YAHOO {- dot -} DE
Date: 2010-02-19

Hello,
sorry for my off topic question.
I found a PEG molecule bound to the active site of my enzyme structure. And I did not expected it there, though I used PEG as precipitant in the crystallization condition. Now I'm wondering how could bind PEG with its hydrophobic nature at all in the hydrophobic active site? It seems that the PEG molecule interacts predominantly with Tryptophans (distance 3.8 A). Did anybody observe similar cases? And which kind of interaction should there be active? I would be grateful for your help,

cheers,

Marek

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: phi/psi angle display in coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2010-02-19

Next message:
Subject: Re: anomalous difference fourier maps
From: Gerard Bricogne gb10 {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-02-19