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Re: [ccp4bb] How to replace 2.45M K/Na phosphate buffer - 2
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
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Subject: Re: How to replace 2.45M K/Na phosphate buffer - 2
From: "Murray, James W" j {- dot -} w {- dot -} murray {- at -} IMPERIAL {- dot -} AC {- dot -} UK
Date: 2007-06-22		Next message:
Subject: Molscript and symbolic label
From: U Sam us_x {- at -} HOTMAIL {- dot -} COM
Date: 2007-06-22


Subject: Re: How to replace 2.45M K/Na phosphate buffer - 2
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2007-06-22

at around 4 molar P-buffer freezes clean.

Best wishes

Kornelius

On Fri, 22 Jun 2007 14:42:00 +0200
Claudia Scotti wrote:
> Sorry: forgotten the pH: the crystals grow between pH 6.5
> and 6.7.
>
> Claudia
>
>
> Claudia Scotti
> Dipartimento di Medicina Sperimentale
> Sezione di Patologia Generale
> Universita' di Pavia
> Piazza Botta, 10
> 27100 Pavia
> Italia
> Tel. 0039 0382 986335/8/1
> Facs 0039 0382 303673
>
> ----------------------------------------
> > Date: Fri, 22 Jun 2007 14:15:47 +0200
> > From: claudiascotti@HOTMAIL.COM
> > Subject: [ccp4bb] How to replace 2.45M K/Na phosphate
> buffer
> > To: CCP4BB@JISCMAIL.AC.UK
> >
> > Dear list,
> >
> > I've crystals in a condition including 2,45 M K/Na
> phosphate buffer for the well condition, and 1 microl
> protein + 0.8 microl Na/K phosphate buffer + 0.25 microl
> 30% 1,6-hexanediol in the drop condition.
> >
> > These crystals dissolve in several cryoprotectant
> solutions and survive only in malonic acid, with which
> they give diffraction at 7-8 A.
> >
> > Among the several different possibilities, I'd like to
> try to optimize the crystals, and one possiblity among
> others would be to change the buffer.
> >
> > Any suggestions, please, about how to replace K/Na
> phosphate? At these concentrations it is acting as a
> buffer but also as a precipitant and I was wondering if
> anybody has experienced nice buffer-salt combinations
> that proved to be useful starting from a similar
> condition.
> >
> > Thanks a lot,
> >
> > Claudia
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Claudia Scotti
> > Dipartimento di Medicina Sperimentale
> > Sezione di Patologia Generale
> > Universita' di Pavia
> > Piazza Botta, 10
> > 27100 Pavia
> > Italia
> > Tel. 0039 0382 986335/8/1
> > Facs 0039 0382 303673
> >
>
_________________________________________________________________
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> >
> http://im.live.com/messenger/im/home/?source=TAGWL_June07
>
>
_________________________________________________________________
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>

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349
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